MSB 2018 offers four stimulating and highly informative short courses for symposium participants. The short courses are lectured by recognized experts in the field and run in parallel on Sunday, February 18 from 9:00 to 13:00 h. The course fee is US$ 100. Participation can be selected during registration for the symposium.

Short-Course 1: Paper-Based Analytical Devices (Room Queluz 7):
presented by Charles S. Henry, Departments of Chemistry, Chemical Engineering, and Biomedical Engineering, Colorado State University, Fort Collins, CO, USA

Paper has long been used as a substrate for chemical measurements, starting from simple litmus paper and advancing to more elegant methods like lateral flow immunoassays. In 2007, Whitesides’ group reported a new use of paper as a substrate for chemical analysis where flow channels were created in paper by adding hydrophobic barriers. In this way, multiplexed chemistry could be carried out in a simple, elegant format. Since this initial work, the field of paper-based analytical devices (also referred to as Lab-on-Paper or Capillarity-Based Microfluidics) has grown exponentially. Growth in this field is the result of the ease of manufacturing and use of the devices. This short course will cover a range of topics within the field, including fundamental device characteristics, fabrication, and applications. A short laboratory demonstration will also be included that provides limited hands on experience with making and using paper-based analytical devices.

Short-Course 2: Strategies to Increase Resolution in Capillary Electrophoresis; (Room Queluz 5):
presented by Dr. Tarso L. Kist, Laboratory of Methods, Institute of Biosciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

The fundamental equation of the resolution between two neighboring peaks in Capillary Electrophoresis contains three terms. The first term expresses the electrophoretic mobility differences, the second term expresses the role of molecular diffusion and the third term contains the square root of the applied electrostatic potential difference. The current general rules used in the practical applications to optimize each term of this fundamental equation are reviewed. New strategies with the potential to bring resolutions to new levels are examined under the light of the best theories of separation sciences.

Short-Course 3: Practical LC-MS Method Development for Small Molecules (Room Queluz 6):
Regina V. Oliveira, Federal University of Sao Paulo, Sao Carlos, Brazil, on behalf of Dr. Perry G. Wang, Office of Regulatory Science, US FDA, USA

This course offers practical training for the practical scientists. It will take the participants step-by-step through the concepts and techniques to develop LC-MS methods. The emphasis is on practical issues associated with developing LC-MS methods for small molecules. It also emphasizes problem-solving skills with examples encountered in the pharmaceutical industry and other fields. This course will provide the participants with an updated overview and a solid working knowledge of LC-MS. The participants will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guides, and key applications. After this course, the participants will be able to independently develop their own LC-MS methods. New technologies and techniques, such as monolithic chromatography and hydrophilic interaction liquid chromatography (HILIC) will be presented. Since some of the participants are from the pharmaceutical industry, which is regulated by GLP and GMP, some regulation and validation concepts will be introduced. 

Short-Course 4: Clinical Metabolomics and Biomarker Discovery using Capillary Electrophoresis-Mass Spectrometry (Room Queluz 1):
presented by Dr. Philip Britz-McKibbin, Department of Chemistry and Chemical Biology, McMaster University, Canada

This short course is aimed at presenting the major technical hurdles associated with biomarker discovery in clinical metabolomics research when using capillary electrophoresis-mass spectrometry (CE-MS). The history of biomarkers and their applications in clinical medicine will first be described, including criteria needed to be satisfied for their qualification and translation to improve patient outcomes. Recent advances in metabolomics for the discovery of new biomarkers of clinical significance will next be discussed, including recommended strategies within a study design, data workflow and statistical approaches to reduce false discoveries. The unique advantages of validated CE-MS protocols in metabolomics research will also be presented, including recent breakthroughs to enhance sample throughput, expand metabolome coverage and identify unknown compounds with quality assurance.